PVDF Transfer Membrane

NuSep PVDF Membrane is ideal for chemiluminescent and colorimetric western blotting: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

• Mechanical strength of PVDF membrane enables multiple reprobing of blots
• Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
• Available in three formats:
  • Convenient precut membrane/blot absorbent filter paper sandwiches
  • Packs of precut membrane-only sheets
  • Economical rolls

Applications

• Transferring proteins from SDS-PAGE gels to membrane
• Immunoblotting target proteins
• Detection by western blotting chemiluminescent or colorimetric methods

Advantages

• High binding affinity
• Low background
• Ready-to-use pre-cut membrane/filter paper sandwiches

Two pore sizes available:

0.45 µm --- most blotting applications
0.20 µm --- lower molecular weights

10 pieces per package

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